67 research outputs found
Assessing the agro-environmental sustainability of organic mixed-crop dairy systems on the basis of a multivariate approach
Sustainable development calls upon the farming sector to commit itself to the transmission of natural resources to future generations. The INRA research team of Mirecourt studies the design of environmentally-friendly farming systems. The design of these systems is based on a multitude of objectives, and their evaluation is determined by a wide range of criteria. This work aims at determining the practical conditions for implementing agricultural systems considered to be sustainable from an environmental point of view. Two organic dairy systems considered to be environmentally friendly ex ante have been designed in partnership with the staff of the INRA research team of Mirecourt. A grazing dairy system and a mixed-crop dairy system are being experimentally tested at the system scale. The two systems have environmental and agricultural objectives. They are managed using multi-objective decision rules and are assessed on their biotechnical and practical properties, using a structured multiyear experimental design, completed by a model-based assessment. Assessment is oriented towards progressive and permanent re-designing of the systems in order to increase their environmental sustainability and feasibility at the practical level. Knowledge acquired from the two prototypes will then have to be validated on commercial farms
Ultrasensitive force detection with a nanotube mechanical resonator
Since the advent of atomic force microscopy, mechanical resonators have been
used to study a wide variety of phenomena, such as the dynamics of individual
electron spins, persistent currents in normal metal rings, and the Casimir
force. Key to these experiments is the ability to measure weak forces. Here, we
report on force sensing experiments with a sensitivity of 12 zN Hz^(-1/2) at a
temperature of 1.2 K using a resonator made of a carbon nanotube. An
ultra-sensitive method based on cross-correlated electrical noise measurements,
in combination with parametric downconversion, is used to detect the
low-amplitude vibrations of the nanotube induced by weak forces. The force
sensitivity is quantified by applying a known capacitive force. This detection
method also allows us to measure the Brownian vibrations of the nanotube down
to cryogenic temperatures. Force sensing with nanotube resonators offers new
opportunities for detecting and manipulating individual nuclear spins as well
as for magnetometry measurements.Comment: Early version. To be published in Nature Nanotechnolog
Palmitoylation mediates membrane association of hepatitis E virus ORF3 protein and is required for infectious particle secretion.
Hepatitis E virus (HEV) is a positive-strand RNA virus encoding 3 open reading frames (ORF). HEV ORF3 protein is a small, hitherto poorly characterized protein involved in viral particle secretion and possibly other functions. Here, we show that HEV ORF3 protein forms membrane-associated oligomers. Immunoblot analyses of ORF3 protein expressed in cell-free vs. cellular systems suggested a posttranslational modification. Further analyses revealed that HEV ORF3 protein is palmitoylated at cysteine residues in its N-terminal region, as corroborated by 3H-palmitate labeling, the investigation of cysteine-to-alanine substitution mutants and treatment with the palmitoylation inhibitor 2-bromopalmitate (2-BP). Abrogation of palmitoylation by site-directed mutagenesis or 2-BP treatment altered the subcellular localization of ORF3 protein, reduced the stability of the protein and strongly impaired the secretion of infectious particles. Moreover, selective membrane permeabilization coupled with immunofluorescence microscopy revealed that HEV ORF3 protein is entirely exposed to the cytosolic side of the membrane, allowing to propose a model for its membrane topology and interactions required in the viral life cycle. In conclusion, palmitoylation determines the subcellular localization, membrane topology and function of HEV ORF3 protein in the HEV life cycle
Frequency fluctuations in silicon nanoresonators
Frequency stability is key to performance of nanoresonators. This stability
is thought to reach a limit with the resonator's ability to resolve
thermally-induced vibrations. Although measurements and predictions of
resonator stability usually disregard fluctuations in the mechanical frequency
response, these fluctuations have recently attracted considerable theoretical
interest. However, their existence is very difficult to demonstrate
experimentally. Here, through a literature review, we show that all studies of
frequency stability report values several orders of magnitude larger than the
limit imposed by thermomechanical noise. We studied a monocrystalline silicon
nanoresonator at room temperature, and found a similar discrepancy. We propose
a new method to show this was due to the presence of frequency fluctuations, of
unexpected level. The fluctuations were not due to the instrumentation system,
or to any other of the known sources investigated. These results challenge our
current understanding of frequency fluctuations and call for a change in
practices
Sox4 participates in the modulation of Schwann cell myelination.
In order to identify new regulators of Schwann cell myelination potentially playing a role in peripheral nervous system (PNS) pathologies, we analysed gene expression profiling data from three mouse models of demyelinating neuropathies and from the developing PNS. This analysis revealed that Sox4, which encodes a member of the Sry-related high-mobility group box protein family, was consistently upregulated in all three analysed models of neuropathy. Moreover, Sox4 showed a peak in its expression during development that corresponded with the onset of myelination. To gain further insights into the role of Sox4 in PNS development, we generated a transgenic mouse that specifically overexpresses Sox4 in Schwann cells. Sox4 overexpression led to a temporary delay in PNS myelination without affecting axonal sorting. Importantly, we observed that, whereas Sox4 mRNA could be efficiently overexpressed, Sox4 protein expression in Schwann cells was strictly regulated. Finally, our data showed that enforced expression of Sox4 in the mouse model for Charcot-Marie-Tooth 4C aggravated its neuropathic phenotype. Together, these observations reveal that Sox4 contributes to the regulation of Schwann cell myelination, and also indicates its involvement in the pathophysiology of peripheral neuropathies
3D characterization of pores in the cortical bone of human femur in the elderly at different locations as determined by synchrotron micro-computed tomography images
International audienceDiaphysis, inferior, and lateral superior regions of the femoral neck are subjected to diverse mechanical loads. Using micro-CT based on synchrotron radiation, three-dimensional morphology and connectivity of the pore network are location dependent, underlying different remodeling mechanisms. INTRODUCTION: The three-dimensional (3D) morphology and connectivity of the pore network at various locations in human femurs subjected to diverse mechanical loads were assessed using micro-CT based on synchrotron radiation. METHODS: The cortex from 20 human femurs (mean age, 78.3 ± 12.4 years) was taken from the diaphysis (D), the inferior (IN), and the lateral superior (LS) regions of the femoral neck. The voxel size of the 3D reconstructed image was 7.5 μm. Cortical thickness and pore volume/tissue volume (Po.V/TV), pore diameter (Po.Dm) and spacing (Po.Sp) were determined. The pore surface/pore volume ratio (Po.S/Po.V), the number of pores (Po.N), the degrees of anisotropy (DA), and the connectivity density (ConnD), the degree of mineralization (DMB) were also determined. RESULTS: The characteristics of the pore network in femoral cortical bone were found to be location dependent. There was greater porosity, Po.Dm, and Po.N, and more large (180-270 μm), extra-large (270-360 μm) and giant pores (>360 μm) in the LS compared to the IN and D. The difference in porosity in between the periosteal and endosteal layers was mostly due to an increase of Po.Dm rather than Po.N. There was a lower DMB of bone in the LS, which is consistent with a higher remodeling rate. CONCLUSION: The results provide evidence for large variations in the structure of the internal pore network in cortical bone. These variations could involve different underlying remodeling mechanisms
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